期刊
PLANT CELL REPORTS
卷 19, 期 5, 页码 459-463出版社
SPRINGER VERLAG
DOI: 10.1007/s002990050756
关键词
Mesembryanthemum crystallinum; Aizoaceae; somatic embryogenesis; halophyte; facultative CAM plant
An efficient plant regeneration procedure has been established from hypocotyl explants of the common ice plant, Mesembryanthemum crystallinum L, a halophytic leaf succulent that exhibits a stress-induced switch from C3 photosynthesis to crassulacean acid metabolism (CAM). Somatic embryos were initiated and developed up to globular and heart stages in Murashige and Skoog (MS) media supplemented with 3% sucrose, 0.6% bacto-agar, 80 mM NaCl, 5 mu M 2,4-D and 1 mu M kinetin. High frequency regeneration occurred when somatic embryos were germinated on media that lacked 2,4-D. High cytokinin treatment suppressed normal growth of embryos and favored abnormal embryo proliferation Without growth regulators, regenerated plants rooted on MS medium with 100% efficiency. Mature, regenerated plants were fertile and morphologically identical to seed-derived plants.
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