期刊
BIOMATERIALS
卷 34, 期 38, 页码 9877-9885出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.08.082
关键词
Coacervate; Polycation; Stromal cell-derived factor (SDF)-1 alpha; Poly(glycerol sebacate); Human progenitor cells; Tissue engineering
资金
- National Heart, Lung, And Blood Institute of the National Institutes of Health [R01HL089658]
- American Heart Association [12EIA9020016]
Host cell recruitment is crucial for vascular graft remodeling and integration into the native blood vessel; it is especially important for cell-free strategies which rely on host remodeling. Controlled release of growth factors from vascular grafts may enhance host cell recruitment. Stromal cell-derived factor (SDF)-1 alpha has been shown to induce host progenitor cell migration and recruitment; however, its potential in regenerative therapies is often limited due to its short half-life in vivo. This report describes a coacervate drug delivery system for enhancing progenitor cell recruitment into an elastomeric vascular graft by conferring protection of SDF-1 alpha. Heparin and a synthetic polycation are used to form a coacervate, which is incorporated into poly(glycerol sebacate) (PGS) scaffolds. In addition to protecting SDF-1 alpha a, the coacervate facilitates uniform scaffold coating. Coacervate-laden scaffolds have high SDF-1 alpha loading efficiency and provide sustained release under static and physiologically-relevant flow conditions with minimal initial burst release. In vitro assays showed that coacervate-laden scaffolds enhance migration and infiltration of human endothelial and mesenchymal progenitor cells by maintaining a stable SDF-1 alpha gradient. These results suggest that SDF-1 alpha coacervate-laden scaffolds show great promise for in situ vascular regeneration. (C) 2013 Elsevier Ltd. All rights reserved.
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