期刊
BIOMATERIALS
卷 34, 期 33, 页码 8269-8278出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.07.072
关键词
Flexor tendon; microRNA; Nanoparticle; Gene delivery; Transfection efficiency
资金
- Hi-Tech Research and Development Program of China (863 Program) [2012AA020502]
- Natural Science Foundation of China [81171457, 21242005]
- Natural Science Foundation of Nantong City [BK2012089]
- Priority of Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Natural Science Foundation of Nantong University [10Z014]
Treatment of the disrupted digital flexor tendon is troublesome because of the lack of sufficient healing capacity and the formation of adhesions. Sustained gene delivery may be a promising approach of modulating gene expression in enhancing tendon healing and decreasing adhesions. In this study, a microRNA-based RNAi plasmid was used to specifically silence the expression of TGF-beta 1 gene associated with scar and adhesion formation in the flexor tendons. The miRNA plasmids were complexed with polylactic-co-glycolic acid (PLGA) nanoparticles to form nanoparticle/TGF-beta 1 miRNA plasmid (nanoparticle/plasmid) complexes. In vitro and in vivo transfection efficiencies experiments against tenocytes revealed that nanoparticle/plasmid complexes have significantly superior transfection efficiency over the lipofectamine/plasmid complexes. The gene and protein expression associated with adhesion of tendon treated with nanoparticle/plasmid complexes were evaluated by real-time PCR and immunoblotting. The grading of adhesions for tendons treated with nanoparticle/plasmid complexes was less severe than that treated with the nanoparticle/mock plasmid complexes. However, the ultimate strength of repaired tendons treated with nanoparticle/plasmid complexes was significantly lower than that of tendons treated with the nanoparticle/mock plasmid complexes. (C) 2013 Elsevier Ltd. All rights reserved.
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