期刊
BIOMATERIALS
卷 34, 期 21, 页码 4926-4935出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.03.020
关键词
Ultrasound; Neural progenitor cell; Microbubble; Stem cell tracking; Gene delivery
资金
- National Institutes of Health [ICMIC P50 CA128346]
- [R21 EB005360]
- [T32 EB005970]
- [R25 CA153915]
Tracking neuroprogenitor cells (NPCs) that are used to target tumors, infarction or inflammation, is paramount for cell-based therapy. We employed ultrasound imaging that can detect a single microbubble because it can distinguish its unique signal from those of surrounding tissues. NPCs efficiently internalized positively charged microbubbles allowing a clinical ultrasound system to detect a single cell at 7 MHz. When injected intravenously, labeled NPCs traversed the lungs to be imaged in the left ventricle and the liver where they accumulated. Internalized microbubbles were not only less sensitive to destruction by ultrasound, but remained visible in vivo for days as compared to minutes when given free. The extended longevity provides ample time to allow cells to reach their intended target. We were also able to transfect NPCs in vitro when microbubbles were preloaded with GFP plasmid only when cells were insonated. Transfection efficiency and cell viability were both greater than 90%. (C) 2013 Elsevier Ltd. All rights reserved.
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