期刊
TRENDS IN BIOTECHNOLOGY
卷 18, 期 4, 页码 147-151出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/S0167-7799(00)01426-8
关键词
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DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers. If single-nucleotide resolution can be achieved, it is possible that nucleic acid sequences can be determined at rates exceeding a thousand bases per second.
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