期刊
MECHANISMS OF DEVELOPMENT
卷 92, 期 2, 页码 239-250出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(00)00245-8
关键词
amphibian; fibronectin matrix; cell migration; early development
资金
- NCI NIH HHS [CA44627] Funding Source: Medline
Fibronectin (FN) is an adhesive extracellular matrix component that is essential for vertebrate development. It forms a fibrillar matrix at the cell surface which controls cell morphology, migration, proliferation, and other important cellular processes. To address specific functions of FN matrix structure during early vertebrate development, we introduced normal and mutant recombinant FNs (recFNs) into the blastocoel cavity of embryos of the amphibian Pleurodeles walfl. Here we show that a native recFN FN(A- B-) as well as recFNs with specific mutations in the cell-binding domain, FN(RGD-) and FN(syn-), or in a FN-binding region, FNL Delta III1, are assembled into fibrillar matrix. A recFN (FN Delta III1-7) that forms a structurally distinct matrix in cultured cells was assembled into aggregates at the cell periphery and was able to inhibit assembly of endogenous amphibian FN matrix in a dose-dependent manner. Cell adhesion, spreading, and migration were perturbed in vitro and in vivo on chimeric matrices containing FN(RGD-), FN(syn-), or FN Delta III1-7 co-assembled with amphibian FN. Developmentally, this perturbation resulted in defects in mesoderm patterning and inhibition of gastrulation. These results indicate that FN matrix fibrillar structure and composition are important determinants of cell adhesion and migration during development. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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