期刊
BIOMATERIALS
卷 34, 期 4, 页码 1344-1353出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.10.047
关键词
Transfection; Polyethylenimine; Non-viral vector; Targeting; Oriented tethering; Epidermal growth factor (EGF)
资金
- Canada Research Chair on Protein-enhanced Biomaterials
- Natural Sciences and Engineering Research Council of Canada
Surface modification of non-viral gene delivery nanocarriers may provide advanced features such as receptor targeting, endosomal escape and nuclear import. We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene expression. Our strategy is based on specific interactions occurring between a coil-tagged ligand and a complementary coil-functionalized nanocarrier. As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated. Coiled-coil-mediated oriented tethering of epidermal growth factor triggered a drastic increase of the internalization rate of the targeted polyplexes. To explore the tunability of our platform, surface density of targeting ligand was varied; our results indicated that the internalization rate varied with the ligand-to-polyplex ratio in a switch mode fashion. This work prefigures possible avenues for our coiled-coil platform in multiplex functionalization to address transient gene expression bottlenecks in recombinant protein production. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
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