期刊
BIOMATERIALS
卷 34, 期 2, 页码 582-597出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.10.001
关键词
PLGA; PEI; SOX9; Cbfa1; C/EBP-alpha
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science, and Technology [2012-0004925, 2012-0006111, 2012R1A1A3003063]
- National Research Foundation of Korea [2012R1A1A3003063] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Wounded tissues and cells may be treated with growth factors and specific genes for the purpose of tissue repair and regeneration. To deliver specific genes into tissues and cells, this study presents the use of fabricated poly (DL-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) complexed with the cationic polymer poly (ethleneimine) (PEI). Through complexation with PEI, several types of genes (SOX9, Cbfa1, and C/EBP-alpha) were coated into PLGA NPs, which enhanced gene uptake into normal human-derived dermal fibroblast cells (NFDHCs) in vitro and in vivo. Several cell types (293T, HeLa, and fibroblast cells) were transfected with fluorescence-tagged PEI/SOX9, PEI/Cbfa1, and PEI/C/EBP-alpha gene-complexed PLGA NPs. The gene and protein expression levels in the cells were evaluated by RT-PCR, real-time quantitative PCR, Western blotting, and confocal laser microscopy. Fibroblast cells encapsulated in fibrin gels were transfected with the gene-complexed NPs plus specific growth factors (TGF-beta 3, BMP-2, or IGF/bFGF), which induced chondrogenesis, osteogenesis, or adipogenesis both in vitro and after transplantation into nude mouse. (c) 2012 Elsevier Ltd. All rights reserved.
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