期刊
BIOMATERIALS
卷 33, 期 33, 页码 8329-8342出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.08.013
关键词
Tissue engineering; Decellularized scaffold; Bone; Human embryonic stem cells; Mineralization
资金
- NIH [DE016525, EB002520]
- New York Stem Cell Foundation [CU09-3055]
- Slovenian Research Agency [P3-0371]
- Aragon Government
- Aragon Health Research Institute
Decellularized bone has been widely used as a scaffold for bone formation, due to its similarity to the native bone matrix and excellent osteoinductive and biomechanical properties. We have previously shown that human mesenchymal and embryonic stem cells form functional bone matrix on such scaffolds, without the use of growth factors. In this study, we focused on differences in bone matrix that exist even among identical harvesting sites, and the effects of the matrix architecture and mineral content on bone formation by human embryonic stem cells (hESC). Mesenchymal progenitors derived from hESCs were cultured for 5 weeks in decellularized bone scaffolds with three different densities: low (0.281 +/- 0.018 mg/mm(3)), medium (0.434 +/- 0.015 mg/mm(3)) and high (0.618 +/- 0.027 mg/mm(3)). The medium-density group yielded highest densities of cells and newly assembled bone matrix, presumably due to the best balance between the transport of nutrients and metabolites to and from the cells, space for cell infiltration, surface for cell attachment and the mechanical strength of the scaffolds, all of which depend on the scaffold density. Bone mineral was beneficial for the higher expression of bone markers in cultured cells and more robust accumulation of the new bone matrix. (C) 2012 Elsevier Ltd. All rights reserved.
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