期刊
BIOMATERIALS
卷 33, 期 31, 页码 7803-7809出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.07.006
关键词
Near-infrared probes; Fluorescence nanoparticles; In vitro imaging; In vivo imaging
资金
- Major Research Plan of the National Natural Science Foundation of China [91027021, 91027041]
- National Natural Science Foundation of China [51173124, 51172151, 50903059, 50825304]
- National Basic Research Program of China (973 Program) [2010CB934500, 2011CB808400]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20093201120020]
- Natural Science Foundation of Jiangsu Province [BK2010003]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
We report a new strategy of using carrier-free pure near-infrared (NIR) dye nanoparticles (NPs) to achieve highly luminescent NIR fluorescent probes for in vitro and in vivo imaging. Bis(4-(N-(2-naphthyl) phenylamino) phenyl)-fumaronitrile (NPAPF) NPs are shown to exhibit favorable biocompatibility, wide-range pH stability (pH 4-10) and much more superior photostability than conventional dyes. Importantly, the combined merits of high dye loading content and aggregation-induced emission enhancement properties, endow the NIR probes with high brightness and a high quantum yield up to 14.9%. The NPAPF NPs can be readily conjugated with folic acid for targeted in vitro cell imaging. Applications of the NPs probes in high efficiency in vivo and ex vivo imaging were successfully demonstrated. Intense fluorescent signals of NPAPF NPs can be distinctly, selectively and spatially resolved in tumor sites with ultrahigh sensitivity, even with 5 ms exposure time, due to the preferentially accumulation of NPs in tumor sites through passive enhanced permeability and retention effect. The totality of results clearly demonstrate the exciting potential of the functionalized NPAPF NPs as a NIR fluorescent probe for in vitro and in vivo imaging and diagnostics. (c) 2012 Elsevier Ltd. All rights reserved.
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