期刊
BIOMATERIALS
卷 33, 期 21, 页码 5325-5332出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.04.004
关键词
Endothelial cells; Collagen scaffold; Perfused vascular construct; 3-D cell culture; 3-D cell printing; Mesoscopic fluorescence molecular tomography
资金
- National Institutes of Health [R21HL102773]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1263455, 1350240] Funding Source: National Science Foundation
Developing methods that provide adequate vascular perfusion is an important step toward engineering large functional tissues. meanwhile, an imaging modality to assess the three-dimensional (3-D) structures and functions of the vascular channels is lacking for thick matrices (>2 similar to 3 mm). Herein, we report on an original approach to construct and image 3-D dynamically perfused vascular structures in thick hydrogel scaffolds. In this work, we integrated a robotic 3-D cell printing technology with a mesoscopic fluorescence molecular tomography imaging system, and demonstrated the capability of the platform to construct perfused collagen scaffolds with endothelial lining and to image both the fluid flow and fluorescent-labeled living endothelial cells at high-frame rates, with high sensitivity and accuracy. These results establish the potential of integrating both 3-D cell printing and fluorescence mesoscopic imaging for functional and molecular studies in complex tissue-engineered tissues. (C) 2012 Elsevier Ltd. All rights reserved.
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