期刊
BIOMATERIALS
卷 33, 期 35, 页码 9135-9147出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.09.007
关键词
Polyglycerols; Topology/architecture of polymers; Blood and cell compatibility; Circulation half-life; Pharmacokinetics and biodistribution; Nano drug delivery systems
资金
- Canadian Institutes of Health Research (CIHR)
- Canada Foundation for Innovation
- Michael Smith Foundation for Health Research (MSFHR)
- CIHR
- MSFHR
- (CIHR/CBS) postdoctoral fellowship in Transfusion Science
- MSFHR postdoctoral fellowship
- CBS
The availability of long circulating, multifunctional polymers is critical to the development of drug delivery systems and bioconjugates. The ease of synthesis and functionalization make linear polymers attractive but their rapid clearance from circulation compared to their branched or cyclic counterparts, and their high solution viscosities restrict their applications in certain settings. Herein, we report the unusual compact nature of high molecular weight (HMW) linear polyglycerols (LPGs) (LPG - 100: M-n - 104 kg mol(-1), M-w/M-n - 1.15) in aqueous solutions and its impact on its solution properties, blood compatibility, cell compatibility, in vivo circulation, biodistribution and renal clearance. The properties of LPG have been compared with hyperbranched polyglycerol (HPG) (HPG-100), linear polyethylene glycol (PEG) with similar MWs. The hydrodynamic size and the intrinsic viscosity of LPG-100 in water were considerably lower compared to PEG. The Mark-Houwink parameter of LPG was almost 10-fold lower than that of PEG. LPG and HPG demonstrated excellent blood and cell compatibilities. Unlike LPG and HPG, HMW PEG showed dose dependent activation of blood coagulation, platelets and complement system, severe red blood cell aggregation and hemolysis, and cell toxicity. The long blood circulation of LPG-100 (t(1/2 beta), 31.8 +/- 4 h) was demonstrated in mice; however, it was shorter compared to HPG-100 (t(1/2 beta), 39.2 +/- 8 h). The shorter circulation half life of LPG-100 was correlated with its higher renal clearance and deformability. Relatively lower organ accumulation was observed for LPG-100 and HPG-100 with some influence of on the architecture of the polymers. Since LPG showed better biocompatibility profiles, longer in vivo circulation time compared to PEG and other linear drug carrier polymers, and has multiple functionalities for conjugation, makes it a potential candidate for developing long circulating multifunctional drug delivery systems similar to HPG. (C) 2012 Elsevier Ltd. All rights reserved.
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