期刊
PLANT CELL REPORTS
卷 19, 期 5, 页码 478-484出版社
SPRINGER
DOI: 10.1007/s002990050759
关键词
Agrobacterium; cotyledonary explant; Glycine max; soybean; transformation
Short-season adapted soybean [Glycine max (L.) Merrill] genotypes (maturity group 0 and 00) were susceptible to Agrobacterium tumefaciens in tumor-formation assays with A. tumefaciens strains A281, C58 and ACH5. The response was bacterial-strain and plant-cultivar dependent. In vitro Agrobacterium-mediated transformation of cotyledonary node explants of these genotypes with A. tumefaciens EHA105/ pB1121 was inefficient but resulted in a transgenic AC Colibri plant carrying a linked insertion of the neomycin phosphotransferase and P-glucuronidase (gus) transgenes. The transgenes were transmitted to the progeny and stable gus expression was detected in the T-7 generation. The low rate of recovery of transgenic plants from the co-cultured cotyledonary explants was attributed to inefficient transformation of regenerable cells, and/or poor selection or survival of such cells and not to poor susceptibility to Agrobacterium, since, depending on the cultivar, explants were transformed at a rate of 27-92%, but transformation events were usually restricted to non-regenerable callus.
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