Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B1 from the chicken duodenum

The ability of Lactobacillus rhamnosus strains GG and LC-705 to remove AFB(1) from the intestinal luminal liquid medium has been tested in vivo using a chicken intestinal loop technique. In this study, the GG strain of L. rhamnosus decreased AFB(1) concentration by 54% in the soluble fraction of the luminal fluid within 1 min. This strain was more efficient in binding AFB(1) compared with L. rhamnosus strain LC-705 (P < 0.05) that removed 44% of AFB(1) under similar conditions. Accumulation of AFB(1) into the intestinal tissue was also determined. There was a 74% reduction in the uptake of AFB(1) by the intestinal tissue, in the presence of L. rhamnosus strain GG compared with 63% and 37% in the case of propionibacterium freudenreichii ssp. shermanii JS and L. rhamnosus strain LC-705, respectively, The complexes formed in vitro between either L. rhamnosus strain GG or L, rhamnosus strain LC-705 and AFB(1) were stable under the luminal conditions for a period of 1 h.