期刊
BIOMATERIALS
卷 33, 期 29, 页码 6943-6951出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.06.057
关键词
Myotube; Myogenic differentiation; Stiffness; Migration
资金
- NIH [DP02OD006460, R21HL106529, P30AR061303]
- Human Frontiers Science Program [RGY0064/2010]
- NSF
- Royal Thai Government Science and Technology
- California Institute for Regenerative Medicine [TB1-01186]
Cell patterning is typically accomplished by selectively depositing proteins for cell adhesion only on patterned regions: however in tissues, cells are also influenced by mechanical stimuli, which can also result in patterned arrangements of cells. We developed a mechanically-patterned hydrogel to observe and compare it to extracellular matrix (ECM) ligand patterns to determine how to best regulate and improve cell type-specific behaviors. Ligand-based patterning on hydrogels was not robust over prolonged culture, but cells on mechanically-patterned hydrogels differentially sorted based on stiffness preference: myocytes and adipose-derived stem cells (ASCs) underwent stiffness-mediated migration, i.e. durotaxis, and remained on myogenic hydrogel regions. Myocytes developed aligned striations and fused on myogenic stripes of the mechanically-patterned hydrogel. ASCs aligned and underwent myogenesis, but their fusion rate increased, as did the number of cells fusing into a myotube as a result of their alignment. Conversely, neuronal cells did not exhibit durotaxis and could be seen on soft regions of the hydrogel for prolonged culture time. These results suggest that mechanically-patterned hydrogels could provide a platform to create tissue engineered, innervated micro-muscles of neural and muscle phenotypes juxtaposed next to each other in order better recreate a muscle niche. (C) 2012 Elsevier Ltd. All rights reserved.
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