期刊
BIOMATERIALS
卷 33, 期 9, 页码 2710-2722出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.11.048
关键词
Nanoparticle; Oxygen-sensing; Ruthenium phosphor; Poly(urethane acrylate nonionomer) (PUAN); Tissue engineering; Vascular oxygen concentration
资金
- NIH [3RO1AR052003-04S1]
- Cornell Center on the Microenvironment and Metastasis from the National Cancer Institute [NCI-U54 CA143876-01, NCI-RC1 CA146065-01]
- Arnold and Mabel Beckman Foundation
- Human Frontiers in Science Programme
- NYSTAR
- Div Of Biological Infrastructure
- Direct For Biological Sciences [0923287] Funding Source: National Science Foundation
We present the development and characterization of nanoparticles loaded with a custom phosphor; we exploit these nanoparticles to perform quantitative measurements of the concentration of oxygen within three-dimensional (3-D) tissue cultures in vitro and blood vessels in vivo. We synthesized a customized ruthenium (Ru)-phosphor and incorporated it into polymeric nanoparticles via self-assembly. We demonstrate that the encapsulated phosphor is non-toxic with and without illumination. We evaluated two distinct modes of employing the phosphorescent nanoparticles for the measurement of concentrations of oxygen: 1) in vitro, in a 3-D microfluidic tumor model via ratiometric measurements of intensity with an oxygen-insensitive fluorophore as a reference, and 2) in vivo, in mouse vasculature using measurements of phosphorescence lifetime. With both methods, we demonstrated micrometer-scale resolution and absolute calibration to the dissolved oxygen concentration. Based on the ease and customizability of the synthesis of the nanoparticles and the flexibility of their application, these oxygen-sensing polymeric nanoparticles will find a natural home in a range of biological applications, benefiting studies of physiological as well as pathological processes in which oxygen availability and concentration play a critical role. (C) 2011 Elsevier Ltd. All rights reserved.
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