期刊
BIOMATERIALS
卷 33, 期 30, 页码 7336-7346出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.06.080
关键词
Corneal tissue engineering; Scaffold; Decellularization; Crosslinking; Genipin
资金
- National High Technology Research and Development Program (863 program) of China [2012AA020507]
- Guangdong Province - Ministry of Education Industry - University - Research Institute Collaboration Project of China [2011B090400353]
- Sun Yat-Sen University 985 Project of China [90035-3283310]
- Fundamental Research Funds of State Key Laboratory of Ophthalmology of China
Acellular porcine corneal stroma (APCS) has been proven to maintain the matrix microenvironment and is therefore an ideal biomaterial for the repair and reconstruction of corneal stroma. This study aims to develop a method to prepare cosmetic corneal lens implants for leukoma using genipin-crosslinked APCS (Gc-APCS). The Gc-APCS was prepared from APCS immersed in 1.0% genipin aqueous solution (pH 5.5) for 4 h at 37 degrees C, followed by lyophilization at -10 degrees C. The color of the Gc-APCS gradually deepened to dark-blue. The degree of crosslinking was 45.7 +/- 4.6%, measured by the decrease of basic and hydroxy amino acids. The porous structure and ultrastructure of collagenous lamellae were maintained, and the porosity and BET SSA were 72.7 +/- 4.6% and 23.01 +/- 3.45 m(2)/g, respectively. The Gc-APCS rehydrated to the physiological water content within 5 min and was highly resistant to collagenase digestion. There were no significant differences in the areal modulus and curvature variation between Gc-APCS and nature porcine cornea. The dark-blue pigments were stable to pH, light and implantation in vivo. Gc-APCS extracts had no inhibitory effects on the proliferation of keratocytes. Corneal neovascularization, graft degradation and corneal rejection were not observed within 6 months. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据