期刊
BIOMATERIALS
卷 33, 期 8, 页码 2449-2461出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.11.074
关键词
Dental follicle cell; Cell sheet technology; Tooth root; Treated dentin matrix; Tooth regeneration
资金
- National Natural Science Foundation of China [30973348]
- National Basic Research Program of China (973 Program) [2010CB944800]
- Key Technology R&D Program of Chengdu Municipal [09GGYB948SF-012]
- International Cooperation Program of Sichuan Province [2010HH0045]
- Key Technology R&D Program of Sichuan Province [2011GZ0115]
- Youth Foundation of Sichuan University [0040305505064]
Stem cell mediated tissue engineering has been acknowledged as a prospective strategy for repairing and replacing damaged and lost tissues. However, the low survival rate of implanted stem cells proves to be a major challenge in the management of transplantation failures. While previous studies have indicated the effectiveness of tissue engineered cell sheets in improving the survival rate of implanted cells, we have recently demonstrated the use of treated dentin matrix (TDM) as a biological scaffold and dental follicle cells (DFCs) as the seeding cells for dentinogenesis and tooth root construction. This study proposes a strategy utilizing TDM with human dental follicle cell sheets (DFCSs) for root regeneration. The biological characteristics and changes of human DFCSs under the effect of TDM were studied with scanning electron microscopy, transmission electron microscopy, immunofluorescence microscopy, immunohistochemistry and quantitative real-time PCR. DFCSs combined with TDM were implanted subcutaneously into the dorsum of mice. Histological examination of the harvested grafts revealed a whirlpool-like alignment of the DFCs in multiple layers that were positive for COLI, integrin beta 1, fibronectin and alkaline phosphatase (ALP), suggestive of the formation of a rich extracellular matrix. DFCSs, under the effect of TDM, highly expressed DMP-1 and bone sialoprotein (BSP), indicating their potential for odontogenesis and osteogenesis. Importantly, in vivo, TDM could induce and support DFCSs to develop new dentin-pulp like tissues and cementum-periodontal complexes that were positive for markers such as DSP, nestin and VIII factors, COLI and cementum attachment protein (CAP), implying successful root regeneration. Therefore, DFCSs combined with TDM may prove to be a better strategy for the construction of tooth root, and is a prospective approach that could be utilized for the treatment of root or tooth defect or loss in future. (C) 2011 Elsevier Ltd. All rights reserved.
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