期刊
BIOMATERIALS
卷 33, 期 4, 页码 1180-1189出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.10.058
关键词
Gold nanoparticles (AuNPs); Therapeutic peptide PMI (p12); Targeted peptide (CRGDK); Neuropilin-1 (Nrp-1) receptor; p53 mechanism; Cancer treatment
资金
- Chinese Natural Science Foundation [30970784, 81171455]
- National Key Basic Research Program of China [2009CB930200]
- Chinese Academy of Sciences (CAS) [07165111ZX]
Functionalization of nanostructures such as gold nanoparticles (AuNPs) with different biological molecules has many applications in biomedical imaging, clinical diagnosis and therapy. Researchers mostly employed AuNPs larger than 10 nm for different biological and medicinal applications in previous studies. Herein, we synthesized a novel small (2 nm) AuNPs, which were functionalized with the therapeutic peptide, PMI (p12), and a targeted peptide, CRGDK for selective binding to neuropilin-1(Nrp-1) receptors which overexpressed on the cancer cells and regulated the process of membrane receptor-mediated internalization. It was found that CRGDK peptides increased intracellular uptake of AuNPs compared to other surface conjugations quantified by ICP-MS. Interestingly, CRGDK functionalized AuNPs resulted in maximal binding interaction between the CRGDK peptide and targeted Nrp-1 receptor overexpressed on MDA-MB-321 cell surface, which improved the delivery of therapeutic P12 peptide inside targeted cells. Au@p12 + CRGDK nanoparticles indicated with highly effective cancer treatment by increasing p53 expression upregulated with intracellular enhanced p12 therapeutic peptide. These results have implications to design and functionalize different molecules onto AuNPs surfaces to make hybrid model system for selective target binding as well as therapeutic effects for cancer treatment. (C) 2011 Elsevier Ltd. All rights reserved.
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