期刊
BIOMATERIALS
卷 33, 期 22, 页码 5603-5609出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.04.044
关键词
Indocyanine green; Near-infrared nanoprobe; Tumor targeting; Molecular imaging; Polymeric nanoparticles
资金
- National Basic Research Program of China (973 program) [2011CB933600]
- National Natural Science Foundation of China [81071249, 20875062, 20905050]
- Hundred Talents program of Chinese Academy of Sciences
- Science and Technology Key Project of Guangdong [2009A030301010]
Indocyanine green (ICG) is a near-infrared (NIR) fluorescence dye for extensive biological application, but limited by its poor aqueous stability in vitro, concentration-dependent aggregation, rapid elimination from the body, and lack of target specificity. In this paper, to overcome these limitations, folate receptor-targeted, ICG dye-doped poly(D,L-lactide-co-glycolid) (PLGA) lipid nanoparticles (FA-ICG-PLGA-lipid NPs) were constructed by a single-step self-assemble and nanoprecipitation method. The prepared FA-ICG-PLGA-lipid NPs exhibited good biocompatibility, monodispersity, excellent NIR penetration ability, significant stability against photobleaching and long circulation time. The intracellular uptake experiment proved the targeting efficacy of the FA-ICG-PLGA-lipid NPs was more effective in folate receptor over-expressing MCF-7 cells than folate receptor negative A549 cells. Furthermore, the in vivo experiments showed the FA-ICG-PLGA-lipid NPs were specifically targeted to the tumor, and its circulation time was much longer than free ICG. These biocompatible and biodegradable NIR-NPs prove a potential application in tumor diagnosis and targeted imaging due to its high aqueous stability, excellent NIR optical properties and significantly targeting property in vivo. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据