期刊
BIOMATERIALS
卷 33, 期 31, 页码 7895-7902出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.07.016
关键词
Rotavirus capsid protein VP4; Iron oxide nanoparticles; Theranostics; Magnetic resonance imaging; Fluorescence imaging; Drug delivery
资金
- Natural Science Foundation of China [21073224]
- Chinese Academy of Sciences [XDA01030200, 2011T1J32]
- National Basic Research Program of China [2010CB933504, 2011CB965004]
- Jiangsu Provincial Fund for Natural Sciences [SBK200922158]
The development of a theranostic nanoplatform based on rotavirus structural protein VP4-coated Fe3O4 nanoparticles (NPs) for dual modality magnetic resonance/fluorescence cellular imaging and drug delivery is reported. VP4 protein was obtained from Escherichia coli approach, and then chemically conjugated to Fe3O4 NPs premodified with meso-2,3-dimercaptosuccinnic acid (DMSA) in the presence of 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide (EDC). Next, the VP4-coated Fe3O4 NPs were loaded with doxorubicin (DOX), a typical anticancer drug, via formation of amide bond through the EDC approach. Prussian blue staining analysis reveals that the VP4-coated Fe3O4 NPs can be internalized efficiently by MA104 and HepG2 cells, thereby significantly improving cellular MRI sensitivity, compared with dextran- and BSA-coated Fe3O4 NPs. In addition, DOX loaded on the VP4-coated Fe3O4 NPs exhibits significant cytotoxicity to the cancer cells (HepG2). The current work provides a general approach toward the rational design and synthesis of a versatile theranostic nanoplatform based on functional protein-coated magnetic NPs with good biocompatibility, biodegradability, and capability of simultaneously performing multimodality imaging and therapy for optimal clinical outcomes. (C) 2012 Elsevier Ltd. All rights reserved.
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