期刊
BIOMATERIALS
卷 33, 期 32, 页码 7993-8002出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.07.064
关键词
Wnt; beta-catenin; MG63; Differentiation; Micro/nano-textured topography
资金
- National Natural Science Foundation of China [81070862, 31170915]
- Hong Kong Research Grants Council (RGC) [CityU 112510]
- Fourth Military Medical University
Wnt/beta-catenin signaling plays a key role in bone formation. To assess the role of this signaling cascade in the response of osteoblasts to the implant topography, human MG63 osteoblasts are cultured on micropitted/nanotubular surface topographies (MNTs) and the transcriptional expressions of Wnt/beta-catenin pathway receptors, activators, and inhibitors are measured. beta-catenin signaling and cell differentiation are studied in the absence and presence of exogenous Dickkopf 1 (Dkk1) on the MNTs and exogenous Wnt3a on a smooth surface. The expressions of the Wnt/beta-catenin pathway receptor low-density lipoprotein receptor-related protein 6 and pathway ligand Wnt3a are up-regulated by the MNTs whereas those of the pathway inhibitors including Dkk1/2 and secreted frizzled-related protein 1/2 are down-regulated by the MNTs, indicating regulation of the Wnt/beta-catenin pathway modulators to activate the pathway. Consequently, the beta-catenin signaling activity is enhanced by the MNTs as well as cell differentiation in terms of osteogenesis-related gene expressions and alkaline phosphatase and collagen products. On the smooth surface, exogenous Wnt3a stimulates beta-catenin signaling and cell differentiation while exogenous Dkk1 attenuates the enhancement by the MNTs. The results explicitly demonstrate that the implant topography regulates the product of the Wnt/beta-catenin pathway modulators from the cells and in turn activates the cell Wnt/beta-catenin pathway promoting osteoblast differentiation. (C) 2012 Elsevier Ltd. All rights reserved.
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