期刊
BIOMATERIALS
卷 32, 期 6, 页码 1526-1535出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.10.021
关键词
Complex tissue engineering; Microencapsulation; Mesenchymal stem cells; Collagen microspheres; Osteochondral interface; Calcified cartilage
资金
- Innovation and Technology Commission
- Hong Kong Government [ITS-133-08]
- University of Hong Kong on Biomedical Engineering and Bionanotechnology
Creating biological interfaces between mechanically dissimilar tissues is a key challenge in complex tissue engineering. An osteochondral interface is essential in preventing mechanical failure and maintaining normal function of cartilage. Despite tremendous efforts in developing osteochondral plugs, formation of the osteochondral interface with proper zonal organization has not yet been reported. Here, we present a mesenchymal stem cell collagen microsphere-based approach for complex tissue engineering and demonstrate in vitro formation of a stem cell-derived osteochondral interface with calcified cartilage interface separating a non-calcified cartilage layer and an underlying bone layer. Cells at the interface region are hypertrophic chondrocytes while the extracellular matrix in this region contains collagen type 11 and X, calcium deposits and vertically running fibers. The simultaneous presence of appropriate medium and configuration during co-culture is necessary for the interface formation. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据