期刊
BIOMATERIALS
卷 32, 期 8, 页码 2141-2148出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.11.053
关键词
Affibody; Bioconjugation; Nanoprobes; HER2; Molecular imaging
资金
- NCI/NIH [R21 CA121842]
- NCI of Cancer Nanotechnology Excellence [U54 CA119367]
This article reports the affibody-based nanoprobes specifically target and image human epidermal growth factor receptor type 2 (HER2)-expressing cells and tumors. The affibody molecules are a promising class of targeting ligands with simple, robust, and precise structure and high affinity. Using near-infrared (NIR) quantum dots (QDs) and iron oxide (IO) nanoparticles as two representative nanomaterials, we designed anti HER2 affibody molecules with a N-terminus cysteine residue (Cysteine-Z(HER2:342)) and precisely conjugated with maleimide-functionalized nanoparticles to make nanoparticle-affibody conjugates. The in vitro and in vivo study showed the conjugates are highly specific to target and image HER2-expressing cells and tumors. This work indicated the nanoparticle-affibody conjugates may be excellent candidates as targeting probes for molecular imaging and diagnosis. (C) 2010 Elsevier Ltd. All rights reserved.
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