期刊
BIOMATERIALS
卷 32, 期 36, 页码 9649-9657出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.08.078
关键词
Angiogenesis; Hydrogel; Degradation; Endothelial cell; Matrix metalloproteinase
资金
- Deutsche Forschungsgemeinschaft (DFG) [WE 2539-7/1, SFB 655, FOR/EXC999]
- Leibniz Association
- European Union
- Bundesministerium fur Bildung, Forschung und Technologie (BMBF) [01 GN 0946]
Cell-responsive degradation of biofunctional scaffold materials is required in many tissue engineering strategies and commonly achieved by the incorporation of protease-sensitive oligopeptide units. In extension of this approach, we combined protease-sensitive and -insensitive cleavage sites for the far-reaching control over degradation rates of starPEG-heparin hydrogel networks with orthogonally modulated elasticity, RGD presentation and VEGF delivery. Enzymatic cleavage was massively accelerated when the accessibility of the gels for proteases was increased through non-enzymatic cleavage of ester bonds. The impact of gel susceptibility to degradation was explored for the 3-dimensional ingrowth of human endothelial cells. Gels with accelerated degradation and VEGF release resulted in strongly enhanced endothelial cell invasion in vitro as well as blood vessel density in the chicken chorioallantoic membrane assay in vivo. Thus, combination of protease-sensitive and -insensitive cleavage sites can amplify the degradation of bioresponsive gel materials in ways that boost endothelial cell morphogenesis. (C) 2011 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据