期刊
BIOMATERIALS
卷 32, 期 6, 页码 1455-1464出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.10.057
关键词
Biosensor; Gold; Nanoparticle; Scaffold; Self assembly; Surface modification
资金
- Bio-oriented Technology Research Advancement Institution (BRAIN) of Japan
- Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN)
- Japan Science and Technology Agency (JST)
- Ministry of Health & Welfare, Republic of Korea [A062254]
- Korea Science and Engineering Foundation [2008-03876]
- National Research Foundation of Korea [2008-03876] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Grants-in-Aid for Scientific Research [23710143, 22650111] Funding Source: KAKEN
To enhance the sensitivities and antigen-binding capacities of immunosensors, oriented immobilization of antibodies on the surface of the sensor chip is critical, but to date, this has not been adequately achieved. We describe a way of adsorbing immunoglobulin (Ig) proteins onto 32-nm bio-nanocapsules (BNCs) through IgG Fc-binding domains derived from Staphylococcus aureus protein A (ZZ-BNC). This arrangement permits similar to 60 molecules of mouse total IgG bind to ZZ-BNC and all the IgG Fv regions to be displayed outwardly for the effective binding of antigens. ZZ-BNCs adsorbed onto the gold surface of the sensor chip of the quartz crystal microbalance (QCM) could markedly enhance the sensitivity and antigen-binding capacity of the chip. On the sensor chip of surface plasmon resonance (SPR), antibodies on the ZZ-BNCs showed higher affinities to each antigen than those on protein A. The BNC-coated sensor chip is very stable, and should prove useful for various immunosensor applications due to oriented immobilization of antibodies. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据