期刊
BIOMATERIALS
卷 32, 期 36, 页码 9899-9907出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2011.09.008
关键词
Liposome; Membrane protein; Proteoliposome; Cadherin; Baculovirus
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18GS0421]
- International Research Center for Molecular Science in Tooth and Bone Diseases at Tokyo Medical and Dental University
- Grants-in-Aid for Scientific Research [18GS0421, 23770222] Funding Source: KAKEN
N-cadherin (CDH2) proteins were reconstituted with liposomes using a baculovirus expression-liposome fusion method. CDH2 budded viruses were fused with giant liposomes containing dioleoylphophogycerol/dioleoylphosphatidylcholine (DOPG/DOPC) at pH 4.5 and the localization of CDH2 on the liposome membrane was observed by confocal laser scanning microscopy. CDH2 liposomes showed Ca2+-dependent association. CDH2-mediated association/dissociation in CDH2 liposomes was specific to Ca2+ and reversible. CDH2-expressing LN-229 cells (human glioblastoma cell) adhered to CDH2 liposomes and small CDH2 liposomes (diameter approximately 150 nm), in particular, were internalized by endocytosis and partly escaped endosomes. Cadherin-containing liposomes show high potential as a new cell-specific proteoliposome. The baculovirus expression-liposome fusion method is useful as a new enabling technology for biomedical applications of functional proteoliposomes. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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