期刊
ANALYTICA CHIMICA ACTA
卷 888, 期 -, 页码 138-145出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.07.026
关键词
Ratiometric fluorescent; Two-photon; Bisulfite; Cell imaging
资金
- Natural Science Foundation of Shandong Province [ZR2014BM004]
- National Natural Science Foundation of China [91313303]
We have developed the first two-photon colorimetric and ratiometric fluorescent probe, BICO, for the detection of bisulfite (HSO3-) in aqueous solution. The probe contains coumarin and benzimidazole moieties and can detect HSO3- based on the Michael addition reaction with a limit of detection 5.3 x 10(-8) M in phosphate-buffered saline solution. The probe was used to detect bisulfite in tap water, sugar and dry white wine. Moreover, test strips were made and used easily. We successfully applied the probe to image living cells, using one-photon fluorescence imaging. BICO overcomes the limitations in sensitivity of previously reported probes and the solvation effect of bisulfite, which demonstrates its excellent value in practical application. (C) 2015 Elsevier B.V. All rights reserved.
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