期刊
BIOMATERIALS
卷 31, 期 7, 页码 1502-1508出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.10.051
关键词
Self-assembly; Nitric oxide; Endothelium; Vascular grafts; Peptide; Stents
资金
- Wallace H. Coulter Foundation
- NIBIB [T32EB004312]
- Caroline P. Ireland Research Scholarship
- NIH [HL71189, HL074391]
Cardiovascular disease is the number one cause of death in the United States. Deployment of stents and vascular grafts has been a major therapeutic method for treatment. However, restenosis, incomplete endothelialization, and thrombosis hamper the long term clinical success. As a solution to meet these current challenges, we have developed a native endothelial ECM mimicking self-assembled nanofibrous matrix to serve as a new treatment model. The nanofibrous matrix is formed by self-assembly of peptide amphiphiles (PAs), which contain nitric oxide (NO) donating residues, endothelial cell adhesive ligands composed of YIGSR peptide sequence, and enzyme-mediated degradable sites. NO was successfully released from the nanofibrous matrix rapidly within 48 h, followed by sustained release over period of 30 days. The NO releasing nanofibrous matrix demonstrated a significantly enhanced proliferation of endothelial cells (51 +/- 3% to 67 +/- 2%) but reduced proliferation of smooth muscle cells (35 +/- 2% to 16 +/- 3%) after 48 h of incubation. There was also a 150-fold decrease in platelet attachment on the NO releasing nanofibrous matrix (470 +/- 220 platelets/cm(2)) compared to the collagen-I (73 +/- 22 x 10(3) platelets/cm2) coated surface. The nanofibrous matrix has the potential to be applied to various cardiovascular implants as a self-assembled coating, thereby providing a native endothelial extracellular matrix (ECM) mimicking environment. (C) 2009 Elsevier Ltd. All rights reserved.
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