期刊
BIOMATERIALS
卷 31, 期 12, 页码 3281-3286出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.01.033
关键词
Enzyme; Electrochemistry; Mesoporous silica nanoparticle; Immunosensor
资金
- National Science Foundation [CMMI-0734881]
Labels based on mesoporous silica nanoparticles (MSN) loaded with mediator thionine (TH), enzyme horseradish peroxidase (HRP) and secondary anti-human IgG antibody (Ab(2)) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. The sensitivity of the sandwich-type immunosensor using MSN-TH-HRP-Ab(2) as labels for human IgG detection was about 100 times higher than that using either MSN-TH-Ab(2) or MSN-HRP-Ab(2) as labels, indicating the high catalytic efficiency of HRP in the presence of mediator TH toward H(2)O(2). The immunosensor using MSN-TH-Ab(2) as labels exhibited a high sensitivity and showed a linear response within the range of 0.01-10 ng/mL human IgG. It also showed good reproducibility and selectivity and acceptable stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据