期刊
BIOMATERIALS
卷 31, 期 8, 页码 2163-2175出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.11.083
关键词
Tendon stem cells; Aligned nanofiber; Differentiation; Tendon regeneration; Mechanotransduction
资金
- National Natural Science Foundation of China [30600301, 30600670, U0672001]
- Zhejiang Province [R206016, 2006C 14024, 2006C 13084]
- Foundation of Zhejiang Provincial Key Medical Discipline
Tendon is a specific connective tissue composed of parallel collagen fibers. The effect of this tissue-specific matrix orientation on stem cell differentiation has not been investigated. This study aimed to determine the effects of nanotopography on the differentiation of human tendon stem/progenitor cells (hTSPCs) and develop a biomimetic scaffold for tendon tissue engineering. The immuno-phenotype of fetal hTSPCs was identified by flow cytometry. The multipotency of hTSPCs toward osteogenesis, adipogenesis, and chondrogenesis was confirmed. Then, the hTSPCs were seeded onto aligned or randomly-oriented Poly (L-lactic acid) nanofibers. Scanning electron micrographs showed that hTSPCs were spindle-shaped and well orientated on the aligned nanofibers. The expression of tendon-specific genes was significantly higher in hTSPCs growing on aligned nanofibers than those on randomly-oriented nanofibers in both normal and osteogenic media. In addition, alkaline phosphatase activity and alizarin red staining showed that the randomly-oriented fibrous scaffold induced osteogenesis, while the aligned scaffold hindered the process. Moreover, aligned cells expressed significantly higher levels of integrin alpha 1, alpha 5 and beta 1 subunits, and myosin II B. In in vivo experiments, the aligned nanofibers induced the formation of spindle-shaped cells and tendon-like tissue. In conclusion, the aligned electrospun nanofiber structure provides an instructive microenvironment for hTSPC differentiation and may lead to the development of desirable engineered tendons. (C) 2009 Elsevier Ltd. All rights reserved.
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