期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 20, 期 7, 页码 2436-2445出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.20.7.2436-2445.2000
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资金
- NCI NIH HHS [P30 CA033572, CA33572] Funding Source: Medline
- NIGMS NIH HHS [GM57484, R01 GM057484] Funding Source: Medline
Eukaryotic genomes contain potentially unstable sequences whose rearrangement threatens genome structure and function. Here we show that certain mutant alleles of the nucleotide excision repair (NER)/TFIIH helicase genes RAD3 and SSL2 (RAD25) confer synthetic lethality and destabilize the Saccharomyces cerevisiae genome by increasing both short-sequence recombination and Ty1 retrotransposition. The rad3-G595R and ssl1-rtt mutations do not markedly alter Ty1 RNA or protein levels or target site specificity. However, these mutations cause an increase in the physical stability of broken DNA molecules and unincorporated Ty1 cDNA, which leads to higher levels of short-sequence recombination and Ty1 retrotransposition. Our results link components of the core NER/TFIIH complex with genome stability, homologous recombination, and host defense against Ty1 retrotransposition via a mechanism that involves DNA degradation.
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