期刊
MODERN PATHOLOGY
卷 13, 期 4, 页码 396-400出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1038/modpathol.3880066
关键词
immunohistochemistry; thyroid; topoisomerase II alpha
类别
Topoisomerase II (topo II) is an enzyme that affects replication, transcription, and chromosome segregation. It serves as a target for several useful anti-chemotherapeutic agents, such as etoposide (VP16) and teniposide (VM26). Monoclonal antibody topo II alpha (Clone JH2.7; Neomarkers, Union City, CA) specifically identifies the alpha isoform of topo II. Using this antibody in an immunohistochemical analysis, we studied differential expression of topo II in a variety of thyroid lesions. The topo II labeling index is defined as the number of topo II staining positive nuclei divided by the total number of tumor cells counted multiplied by 100. An average of 1000 cells were counted in each case. The average labeling indexes for anaplastic carcinoma (7.8), tall cell variant of papillary carcinoma (4.8), follicular carcinoma (2.6), Hurthle cell carcinoma (3.4), and medullary carcinoma (2.4) were much higher than for papillary carcinoma (0.76), follicular adenoma (0.65), Hurthle cell adenoma (0.32), and normal thyroid (0.1). This study suggests that immunohistochemical analysis of topo LI correlates with thyroid tumor histology; it is more frequently expressed in tumors that are associated with aggressive clinical behavior. It may help to define a role for anti-topoisomerase drugs in treatment of aggressive thyroid neoplasms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据