期刊
BIOMATERIALS
卷 31, 期 11, 页码 3063-3070出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.12.054
关键词
Titanium dioxide nanoparticles; Neural stem cells; Differentiation; Protein-protein interaction; Proteomics
资金
- China Ministry of Science and Technology 973 Project [2006CB705604, 2006CB500702]
- National Science Foundation of China [30570590, 20871081]
- Shanghai Commission of Education Science and Technology Innovation Fund [08ZZ41]
- Shanghai Committee of Science and Technology [09JC1406600]
The effects of titanium dioxide (TiO2) nanoparticles (NPs) on the differentiation of neural stem cells are reported. Our findings indicate that TiO2 NPs lead to a differentiational tendency towards neurons from neural stem cells, suggesting TiO2 NPs might be a beneficial inducer for neuronal differentiation. To insight into the possible molecular mechanism of the neuronal differentiation, we conducted a protein-protein interaction network (PIN) analysis. To this end, a global mapping of target proteins induced by TiO2 NPs was first made by a 2-dimensional electrophoresis analysis. Results showed that 9 proteins were significantly changed and then they were subjected to the mass spectrometric assay. All 9 identified proteins are involved in signal, molecular chaperones, cytoskeleton, and nucleoprotein. Further, based on our experimental data and DIP, IntAct-EBI, GRID database, a protein-protein interaction network was constructed, which provides highly integrated information exhibiting the protein-protein interaction. By analysis of the gene expression, the signal pathway involving Cx43 phosphotylation, which is negatively regulated by the protein kinase C epsilon (PKC epsilon), is demonstrated. It is inferred that PKCE plays a pivotal negative role in the neuronal differentiation of stem neural cells in response to the TiO2 NPs exposure. (C) 2009 Elsevier Ltd. All rights reserved.
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