期刊
BIOMATERIALS
卷 31, 期 8, 页码 2243-2251出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.11.094
关键词
Three-dimensional culture; Scaffold; Leukaemia culture; Haematopoiesis
资金
- Richard Thomas Leukaemia Fund
- Lady Tata Memorial Trust
- Northwick Park Hospital Leukaemia Research Trust Fund
Acute myeloid leukaemia (AML) is a cancer of haematopoietic cells that develops in three-dimensional (3-D) bone marrow niches in vivo. The study of AML has been hampered by lack of appropriate ex vivo models that mimic this microenvironment. We hypothesised that fabrication and optimisation of suitable biomimetic scaffolds for culturing leukaemic cells ex vivo might facilitate the study of AML in its native 3-D niche. We evaluated the growth of three leukaemia subtype-specific cell lines, K-562, HL60 and Kasumi-6, on highly porous scaffolds fabricated from biodegradable and non-biodegradable polymeric materials, such as poly (L-lactic-co-glycolic acid) (PLGA), polyurethane (PU), poly (methylmethacrylate), Poly (D, L-lactade), poly (caprolactone), and polystyrene. Our results show that PLGA and PU supported the best seeding efficiency and leukaemic growth. Furthermore, the PLGA and PU scaffolds were coated with extracellular matrix (ECM) proteins, collagen type I (62.5 or 125 mu g/ml) and fibronectin (25 or 50 mu g/ml) to provide biorecognition signals. The 3 leukaemia subtype-specific lines grew best on PU scaffolds coated with 62.5 mu g/ml collagen type I over 6 weeks in the absence of exogenous growth factors. In conclusion, PU-collagen scaffolds may provide a practical model to study the biology and treatment of primary AML in an ex vivo mimicry. (C) 2009 Elsevier Ltd. All rights reserved.
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