期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 11, 期 4, 页码 292-300出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S1044-0305(99)00148-8
关键词
-
Disaccharides tagged with p-aminobenzoic acid (ABA) were separated by capillary electrophoresis (CE) and analyzed on-line with negative ion electrospray ionization tandem mass spectrometry (ESI/MS/MS). The formation of glycosylamine instead of reductive amination was selected as the derivatization reaction. In negative ion ESI, the glycosylamine approach provides more information on linkage and anomeric configuration than reductive amination. In CE analysis of ABA-labeled disaccharides, alpha-cyclodextrin (CD) was found to play a crucial role in the separation of linkage isomers. Although ammonium acetate/alpha-CD provided the best resolution of linkage isomers, the berate buffer was superior to alpha-CD in the separation of disaccharides with the same linkage but different anomeric configuration and/or monosaccharide composition. Both alpha-CD and berate suppressed the ion signal in ESI, and operational conditions were successfully obtained using 10 mM (alpha-CD or 10 mM berate. CT Am Soc Mass Spectrom 2000, 11, 292-300) (C) 2000 American Society for Mass Spectrometry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据