期刊
BIOMATERIALS
卷 31, 期 34, 页码 8911-8920出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.07.110
关键词
Bioreactor; ECM (extracellular matrix); Electrospun scaffold; Bioactivity; Cartilage tissue engineering; Mesenchymal stem cell
资金
- National Institutes of Health [R01 AR57083]
In this study, electrospun poly(epsilon-caprolactone) (PCL) microfiber scaffolds, coated with cartilaginous extracellular matrix (ECM), were fabricated by first culturing chondrocytes under dynamic conditions in a flow perfusion bioreactor and then decellularizing the cellular constructs. The decellularization procedure yielded acellular PCL/ECM composite scaffolds containing glycosaminoglycan and collagen. PCL/ECM composite scaffolds were evaluated for their ability to support the chondrogenic differentiation of mesenchymal stem cells (MSCs) in vitro using serum-free medium with or without the addition of transforming growth factor-beta 1 (TGF-beta 1). PCL/ECM composite scaffolds supported chondrogenic differentiation induced by TGF-beta 1 exposure, as evidenced in the up-regulation of aggrecan (11.6 +/- 3.8 fold) and collagen type II (668.4 +/- 317.7 fold) gene expression. The presence of cartilaginous matrix alone reduced collagen type I gene expression to levels observed with TGF-beta 1 treatment. Cartilaginous matrix further enhanced the effects of growth factor treatment on MSC chondrogenesis as evidenced in the higher glycosaminoglycan synthetic activity for cells cultured on PCL/ECM composite scaffolds. Therefore, flow perfusion culture of chondrocytes on electrospun microfiber scaffolds is a promising method to fabricate polymer/extracellular matrix composite scaffolds that incorporate both natural and synthetic components to provide biological signals for cartilage tissue engineering applications. (C) 2010 Elsevier Ltd. All rights reserved.
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