期刊
BIOMATERIALS
卷 31, 期 15, 页码 4376-4381出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.02.017
关键词
Electrospun scaffold; Endothelial regeneration; Anti-thrombotic vascular graft
资金
- National Science Foundation (NSF) [DMR-0402891]
The capacity of the luminal layer of an electrospun bi-layer scaffold composed of gelatin, elastin, polycaprolactone (PCL), and poliglecaprone (PGC) to promote endothelial regeneration was investigated using human aortic endothelial cells (HAECs). HAECs of different densities were cultured on a thin film of the luminal layer of the scaffold mounted on a cell crown for desired periods. Fluorescent images showed that HAECs formed a mono-layer within 24 h after having successfully adhered to the scaffold's surface. Scanning electron microscopy (SEM) revealed a satisfactory coverage by the HAECs. Death rates of HAECs populations determined by fluorescent staining were below 5% within the initial 3 days while the profile of proliferation exhibited an exponential increase within 11 days as determined by the 3-[4,5-dimethyl (thiazol-2y1)-3,5-diphery] tetrazolium bromide (MTT) assay. The functionalities of the endothelial monolayer were probed by ZO-1 staining for tight junction formation, by 6-keto-PGF(1 alpha), assay for prostacyclin (PGI(2)) secretion, and by human platelets for its anti-thrombotic capability. The results indicated that the regenerated endothelium possessed normal functions associated with native endothelium. This study suggests that this electrospun bi-layer scaffold is a promising candidate for cardiovascular grafting for its capability of promoting the regeneration of a functional endothelium to prevent blood clotting in small diameter grafts. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据