期刊
BIOMATERIALS
卷 31, 期 33, 页码 8651-8658出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.07.089
关键词
Inverse opal scaffolds; Photoacoustic microscopy; Melanoma; Tissue engineering; Biomedical imaging
资金
- NIH [DP1 OD000798, R01 EB000712, R01 NS46214, R01 EB008085, U54 CA136398]
- Washington University in St. Louis
- NSF [ECS-0335765]
- Microphotoacoustics, Inc.
- Endra, Inc.
Visualizing cells in three-dimensional (3D) scaffolds has been one of the major challenges in tissue engineering. Most current imaging modalities either suffer from poor penetration depth or require exogenous contrast agents. Here, we demonstrate photoacoustic microscopy (PAM) of the spatial distribution and temporal proliferation of cells inside three-dimensional porous scaffolds with thicknesses over 1 mm. Specifically, we evaluated the effects of seeding and culture methods on the spatial distribution of melanoma cells. Spatial distribution of the cells in the scaffold was well-resolved in PAM images. Moreover, the number of cells in the scaffold was quantitatively measured from the as-obtained volumetric information. The cell proliferation profile obtained from PAM correlated well with what was obtained using the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (C) 2010 Elsevier Ltd. All rights reserved.
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