期刊
BIOMATERIALS
卷 31, 期 30, 页码 7827-7835出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2010.06.052
关键词
Biomimetic layer; Langmuir-Blodgett film; Cytochrome c; beta-Nicotinamide adenine dinucleotide; Phospholipid
资金
- Ministry of Environment [091-082-078]
- National Research Foundation of S. Korea [2009-007-9376]
Biomimetic layers triggering the redox process of cytochrome c (cyt c) by beta-nicotinamide adenine dinucleotide (NADH) were fabricated and applied for the detection of NADH. A probe was constructed based on a conducting polymer (poly-5,2':5',2 ''-terthiophene-3'-carboxylic acid, poly-TTCA) formed on the Au nanoparticles, which were deposited on a screen-printed carbon electrode; the probe was modified with biomaterials including cyt c, lipids (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and cardiolipin), and ubiquinone, which are involved in electron transfer sequence in the cell membrane. To eliminate affection of foreign biological species, we assembled a lipid bilayer using the Langmuir-Blodgett technique by controlling the density of the outward lipid layer. The characteristics of the biomimetic layers were investigated by cyclic voltammetry, impedance spectrometry, quartz crystal microbalance, and X-ray photoelectron spectroscopy. The electron transfer process triggered by the presence of NADH was used to determine the NADH concentration. L-ascorbic acid, uric acid, and NADPH did not show any affection during the detection of NADH at the density controlled-lipid bilayer. Real sample analysis was successfully performed to evaluate the reliability of the proposed biomimetic probe. (C) 2010 Elsevier Ltd. All rights reserved.
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