期刊
PLANT DISEASE
卷 84, 期 4, 页码 429-436出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PDIS.2000.84.4.429
关键词
bitter gourd; RFLP; 16S rRNA
Chayote (Sechium edule) (Cucurbitaceae), also known as vegetable pear, mirliton, or mango squash, is a commercially important vegetable crop in Brazil, where it is affected by chayote witches'-broom disease. Affected plants exhibit witches'-broom growths and other symptoms characteristic of plant diseases caused by phytoplasmas. Since previous electron microscopic studies revealed the association of a phytoplasma with chayote witches'-broom, the present work was aimed at detecting and classifying the phytoplasma that may be the causal agent of the disease. Strains of a phytoplasma belonging to group 16SrIII (X-disease phytoplasma group) were discovered in chayote affected by witches'-broom disease and in diseased plants of Momordica charantia that were growing as weeds in fields of chayote in Brazil. On the basis of results from restriction fragment length polymorphism and nucleotide sequence analyses of 16S rDNA, tbe phytoplasma was classified in a new subgroup, designated subgroup III-J. This classification was supported by a phylogenetic tree constructed by the Neighbor-Joining method.
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