Fatigue and human umbilical cord stem cell seeding characteristics of calcium phosphate-chitosan-biodegradable fiber scaffolds

Calcium phosphate cement (CPC) has in situ-setting ability and bioactivity, but the brittleness and low strength limit CPC to only non-load-bearing bone repairs. Human umbilical cord mesenchymal stem cells (hUCMSCs) can be harvested without an invasive procedure required for the commonly studied bone marrow MSCs. However, little has been reported on hUCMSC delivery via bioactive scaffolds for bone tissue engineering. The objectives of this study were to develop CPC scaffolds with improved resistance to fatigue and fracture, and to investigate hUCMSC delivery for bone tissue engineering. In fast fracture, CPC with 15% chitosan and 20% polyglactin fibers (CPC-chitosan-fiber scaffold) had flexural strength of 26 MPa, higher than 10 MPa for CPC control (p < 0.05). In cyclic loading, CPC-chitosan-fiber specimens that survived 2 x 10(6) cycles had the maximum stress of 10 MPa, compared to 5 MPa of CPC control. CPC-chitosan-fiber specimens that failed after multiple cycles had a mean stress-to-failure of 9 MPa, compared to 5.8 MPa for CPC control (p < 0.05). hUCMSCs showed excellent viability when seeded on CPC and CPC-chitosan-fiber scaffolds. The percentage of live cells reached 96-99%. Cell density was about 300 cells/mm(2) at day 1; it proliferated to 700 cells/mm(2) at day 4. Wst-1 assay showed that the stronger CPC-chitosan-fiber scaffold had hUCMSC viability that matched the CPC control (p > 0.1). In summary, this study showed that chitosan and polyglactin fibers substantially increased the fatigue resistance of CPC, and that hUCMSCs had excellent proliferation and viability on the scaffolds. (C) 2009 Elsevier Ltd. All rights reserved.