期刊
BIOMATERIALS
卷 30, 期 9, 页码 1756-1762出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2008.12.018
关键词
Titanium particles; Osteoclast; NFATc1; Cytokines; Osteolysis
资金
- National Natural Science Foundation of China [30772184]
- Support Program for Discipline Leaders in Shanghai [06XD14033]
Osteoclastogenesis induced by particulate wear debris is a major pathological factor contributing to periprosthetic osteolysis. Although the nuclear factor of activated T cells c1 (NFATc1) is known to be involved in osteoclast differentiation, its effect on osteoclastogenesis in response to wear particles remains unclear. In the present study, we investigated the role of NFATc1 in the regulation of osteoclast differentiation from bone marrow macrophages (BMMs) stimulated with titanium (Ti) particles. The results showed that Ti particles could stimulate BMMs to produce proinflammatory cytokines (tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6) and differentiate into multinucleated osteoclasts in the presence of receptor activator of nuclear factor-kappa B ligand (RANKL). NFATc1 was expressed in BMMs and multinucleated cells cultured with Ti particles and RANKL Inactivation of NFATcl by 11R-VIVIT peptide potently impeded the Ti particle-induced osteoclastogenesis. 11R-VIVIT peptide does not have toxic effect on BMMs. Based on these data, we conclude that inactivation of NFATcl by VIVIT peptide would provide a promising therapeutic target for the treatment of periprosthetic osteolysis. (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据