期刊
BIOMATERIALS
卷 30, 期 20, 页码 3405-3414出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.02.046
关键词
Biodegradation; Cell adhesion; Elastomer; Stem cell; Nerve tissue engineering; Ophthalmology
资金
- NIH [DE013023, HL060435]
- National Eye Institute Award [T32 EY07145-06]
- National Eye Institute [1 F32 EY018285-01]
Retinal degenerations cause permanent visual loss and affect millions world-wide. Presently, a novel treatment highlights the potential of using biodegradable polymer scaffolds to induce differentiation and deliver retinal progenitor cells for cell replacement therapy. In this study, we engineered and analyzed a micro-fabricated polymer, poly(glycerol sebacate) (PGS) scaffold, whose useful properties include biocompatibility, elasticity, porosity, and a microtopology conducive to mouse retinal progenitor cell (mRPC) differentiation. In vitro proliferation assays revealed that PGS held up to 86,610 (+/- 9993) mRPCs per square millimeter, which were retained through simulated transplantations. mRPCs adherent to PGS differentiated toward mature phenotypes as evidenced by changes in mRNA, protein levels, and enhanced sensitivity to glutamate. Transplanted composites demonstrated long-term mRPC survival and migrated cells exhibited mature marker expression in host retina. These results suggest that combining mRPCs with PGS scaffolds for subretinal transplantation is a practical strategy for advancing retinal tissue engineering as a restorative therapy. (c) 2009 Elsevier Ltd. All rights reserved.
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