期刊
BIOMATERIALS
卷 30, 期 29, 页码 5631-5638出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.07.026
关键词
Stem cell differentiation; Gold nanoparticles; hESCs; Gene modified hESCs; Electrical stimulation
资金
- Ministry of Education, Science and Technology, Republic of Korea [SC-1140]
- National Research Foundation of Korea [12-2008-09-004-00] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
To encourage stem cell differentiation, gold nanoparticles (20 nm) were used to deliver electrical stimulation to human embryonic stem cells (hESCs) in vitro. Nano-structured gold nanoparticles were designed by coating the surface of culture dishes with gold nanoparticles using a layer-by-layer (LBL) system. In this method, gold nanoparticles were continuously coated onto dishes by SEM analysis. Evaluation of gene modified hESCs that were subsequently attached onto fibronectin-coated gold nanoparticles revealed that the un-differentiation marker, Oct-4, was no longer present following electrical stimulation. In addition, the osteogenic markers of collagen type I and Cbfa1 increased in response to electrical stimulation, while those of hESCs were not observed without electrical stimulation. (C) 2009 Published by Elsevier Ltd.
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