期刊
BIOMATERIALS
卷 30, 期 27, 页码 4522-4532出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.05.021
关键词
Tissue engineering; Silk scaffold; Apatite; Bone morphogenetic proteins; Bone marrow stromal cells
资金
- National Natural Science Foundation of China [30400502, 30772431]
- Science and Technology Commission of Shanghai Municipality [07DZ22007, 08410706400, 08JC1414400, 08DZ2271100, S30206]
- Shanghai Rising-star Program [05QMX1426, 08QH14017]
- NIH [DE16710]
- [NCET-08-0353]
Premineralized silk fibroin protein scaffolds (mSS) were prepared to combine the osteoconductive properties of biological apatite with aqueous-derived silk scaffold (SS) as a composite scaffold for bone regeneration. The aim of present study was to evaluate the effect of premineralized silk scaffolds combined with bone morphogenetic protein-2 (BMP-2) modified bone marrow stromal cells (bMSCs) to repair mandibular bony defects in a rat model. bMSCs were expanded and transduced with adenovirus AdBMP-2, AdLacZ gene in vitro. These genetically modified bMSCs were then combined with premineralized silk scaffolds to form tissue-engineered bone. Mandibular repairs with AdBMP-2 transduced bMSCs/mSS constructs were compared with those treated with AdLacZ-transduced bMSCs/mSS constructs, native (nontransduced) bMSCs/mSS constructs and mSS alone. Eight weeks after post-operation, the mandibles were explanted and evaluated by radiographic observation, micro-CT, histological analysis and immunohistochemistry. The presence of BMP-2 gene enhanced tissue-engineered bone in terms of the most new bone formed and the highest local bone mineral densities (BMD) found. These results demonstrated that premineralized silk scaffold could serve as a potential substrate for bMSCs to construct tissue-engineered bone for mandibular bony defects. BMP-2 gene therapy and tissue engineering techniques could be used in mandibular repair and bone regeneration. (C) 2009 Elsevier Ltd. All rights reserved.
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