期刊
BIOMATERIALS
卷 30, 期 31, 页码 6295-6308出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2009.08.011
关键词
Thermosensitive; Injectable; Polyphosphazene-RGD conjugate; Mesenchymal stem cells (MSCs); Osteogenic differentiation; Hydrogel
资金
- Ministry of Education, Science and Technology in Korea
- Korea Institute of Science and Technology (KIST)
An injectable and thermosensitive poly(organophosphazene)-RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)GRGDS conjugates existed in an injectable fluid state at room temperature and immediately formed a hydrogel at body temperature. The rabbit mesenchymal stem cells (rMSCs) on the polymer-GRGDS conjugate (conjugate 1-2,0.05 mol fraction as GRGDS) hydrogel constructs using an injection method into a nude mouse were proved to express markers at mRNA level for all stages towards osteogenesis and mainly a sharp increase of osteocalcin (OCN, a typical late osteogenic differentiation marker) levels at 4th week post-induction indicated that the maturation process has started within this period. By histological and immunohistochemical evaluations, significantly high mineralization level by calcium contents was detected qualitatively and collagen type I (Col I), a major characteristic marker protein, was mainly and highly expressed by the rMSCs cultivated in the polymer-GRGDS conjugate hydrogel constructs formed into the nude mouse. The results suggest that the poly(organophosphazene)-GRGDS conjugate to enhance biofunctionality hold a promise for cell delivery material to induce osteogenic differentiation of MSC for enhancing ectopic bone formation. (C) 2009 Elsevier Ltd. All rights reserved.
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