期刊
BIOMATERIALS
卷 30, 期 6, 页码 1066-1070出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2008.10.027
关键词
Cell culture; Cell spreading; Cell viability; Plasma polymerisation
资金
- Royal Society and the Medical Research Council UK [G0300296]
- Biotechnology and Biological Sciences Research Council [BB/D014638/1] Funding Source: researchfish
- Medical Research Council [G117/423, G0300296] Funding Source: researchfish
- BBSRC [BB/D014638/1] Funding Source: UKRI
- MRC [G0300296, G117/423] Funding Source: UKRI
Plasma polymer surfaces were fabricated such that the cell response to a range of carboxylic acid concentrations on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised octadiene (OD) to a more hydrophilic plasma polymerised acrylic acid (AA) were formed on glass coverslips. Surface characterisation of the chemical gradients was performed using X-ray photoelectron spectroscopy to determine elemental composition. Following culture of E14 and RI mouse embryonic stem cells (mES) in differing culture media, cell pluripotency was determined by alkaline phosphatase staining. The results demonstrate that for these cell lines the capacity for self-renewal is maintained if the cells are restricted in their spreading to <120 mu m(2). (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据