期刊
MOLECULAR PLANT-MICROBE INTERACTIONS
卷 13, 期 4, 页码 394-401出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI.2000.13.4.394
关键词
type II protein secretion
Xanthomonas oryzae pv, oryzae (Xoo) causes bacterial leaf blight, a serious disease of rice. A virulence- and xylanase-deficient mutant of Xoo was isolated following ethyl methane sulfonate (EMS) mutagenesis. A cosmid clone that restored virulence and xylanase secretion was obtained from a genomic library by functional complementation. Transposon mutagenesis and marker exchange studies revealed genes on the cloned DNA that were required for xylanase production and virulence. Sequence analysis with transposon-specific primers revealed that these genes were homologues of xps F and xps D, which encode components of a protein secretion system in Xanthomonas campestris pv, campestris. Enzyme assays showed xylanase accumulation in the periplasmic space and cytoplasm of the xps F mutant and the complementing clone restored transport to the extracellular space.
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