期刊
BIOMATERIALS
卷 29, 期 10, 页码 1356-1366出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2007.11.035
关键词
nafion; surface modification; HEMA; tetraglyme; protein adsorption
资金
- NIBIB NIH HHS [R21 EB000987, R21 EB000987-01S1, 3R21 EB00987-S1, R21 EB000987-01, R21 EB000987-03, P41 EB002027, 1R21EB00987, R21 EB000987-02, P41 EB002027-19] Funding Source: Medline
- NIDCR NIH HHS [R01 DE018701] Funding Source: Medline
Nafion (TM) is the membrane material preferred for in situ glucose sensors. Unfortunately, surface properties of Nation promote random protein adsorption and eventual foreign body encapsulation thus leading to loss of glucose signal over time. Here we detail surface modifications made by RF plasma deposition to Nafion with the intent to prevent random protein adsorption while providing enough functional sites (hydroxyl groups) to bind a biologically active peptide known to induce cellular adhesion (YRGDS). Nafion surfaces were modified by RF plasma polymerizing five different combinations of (1) tetraethylene glycol dimethyl ether (tetraglyme) and (2) 2-hydroxyethyl methacrylate (HEMA): pure tetraglyme, 2.5% HEMA with 97.5% tetraglyme, 5% HEMA with 95% tetraglyme, 10% HEMA with 90% tetraglyme, and pure HEMA. Resultant surfaces were characterized by XPS (low and high resolution), dynamic contact angle, and atomic force microscopy. Protein adsorption and retention was determined and correlated to surface layer composition. The ability to bind a cell adhesion peptide was also determined and correlated well with surface layer composition. (c) 2007 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据